Development of Assay System for Factors using Whole Cell CulturesImmunoglobulin Production of Mouse Splenocytes Regulatory
نویسندگان
چکیده
We tried to establish an assay system £or screening and assessment of immunoregulatory factors using whole cell cultures of mouse splenocytes and found that splenic adhesiye cells markedly increased immunogobulin (Ig) production of splenocytes. In the absence of adhesiye cells, lipopolysaccharides, pokeweed mitogen, and phytohemagglutinin stimulated the prod"ction of IgA, IgG, and IgM in a class-depende"t manner. Adhesive cells increased more markedly Ig production of splenocytes stimulated with these mitogens. When mouse sp)enocytes were cti}tured with milk proteins in the absellce of adhesive cells, lactoferrin, fi-]actoglobulin, a-casein, and fi-casein stimulated IgA and IgG production. Adhesive cells increased IgA production of
منابع مشابه
Effect of serum starvation stress on the mouse spleen mononuclear cells mixed culture: Introducing a new immunomodulatory method
Introduction: Bone marrow and immune cell transplants are a common method of treating some blood diseases around the world. However, due to the malfunction of the immune system, its use is not always effective. In this study, we evaluated the potential of culture stress in serum-free medium in regulating the immune system. Materials and Methods: Spleen immune cells were isolated from Balb/C and...
متن کاملModulation of CD4+ T Cell Subsets by Euphorbia microciadia and Euphorbia osyridea Plant Extracts
Background: Euphorbia plants are traditionally used in folk medicine for infections, inflammation, and cancer. Objectives: To investigate the effects of the butanolic extracts of Euphorbia micorociadia and Euphorbia osyridea on specific transcription factors and cytokines expression of T cell subsets. Methods: Activated mouse splenocytes were cultured in the presence of non-cytotoxic concentrat...
متن کاملComparative Assessment of the Whole-cell Pertussis Vaccine Potency Using Serological and Intracerebral Mouse Protection Methods
One of the most important QC tests of whole-cell pertussis vaccine (WCPV) is potency test. In this regard, mouse protection test (MPT) is the current potency method, which is associated with severe animal distress and large variability in results. The purpose of this study was to assess Pertussis Serological Potency Test (PSPT) as a serological alternative method to intracerebral challenge in M...
متن کاملEffect of Different Concentrations of Forskolin Along with Mature Granulosa Cell Co-Culturing on Mouse Embryonic Stem Cell Differentiation into Germ-Like Cells
Background: Germ cell development processes are influenced by soluble factors and intercellular signaling events between them and the neighboring somatic cells. More insight into the molecular biology of the germ cell development from embryonic stem (ES) cells and investigation of appropriate factors, specifically those targeting differentiation process, is of great importance. In this study, w...
متن کاملDevelopment of Clay Foam Ceramic as a Support for Fungi Immobilization to Biodiesel Production (RESEARCH NOTE)
Biodiesel is an attractive alternative fuel because of its nontoxicity and biodegradability properties. Biodiesel is produced through transesterification of vegetable oils’ triglyceride. It is obtained from vegetable oils or fats either by chemical or enzyme-catalyzed transesterification with methanol or ethanol. By using whole-cell biocatalyst immobilized within biomass support particles (BSPs...
متن کامل